Fig. 7
From: Superficially serrated adenoma: a proposal for a novel subtype of colorectal serrated lesion
RSPO expression in superficially serrated adenoma-associated traditional serrated adenomas. a–c RT-PCRs targeting the PTPRK exon 1-RSPO3 (a), PTPRK exon 6-RSPO3 (b), and PTPRK exon 7-RSPO3 (c) fusions of superficially serrated adenoma and traditional serrated adenoma components of superficially serrated adenoma-associated traditional serrated adenomas. ACTB served as a positive control. d Sanger sequencing results for the RSPO fusion junctions and KRAS. The PTPRK exon 7-RSPO3 fusion product was sequenced using a reverse primer. e, f Quantitative RT-PCR analysis of the RSPO2 exons 1 and 2 and exons 3 and 4 (e) and RSPO3 exons 3 and 4 (f) junctions of the superficially serrated adenoma and traditional serrated adenoma components of superficially serrated adenoma-associated traditional serrated adenomas. Expression levels are indicated relative to normal colon mucosa. Notice that RSPO3 overexpression was detected in both components in all the lesions with RSPO fusions and/or overexpression. T traditional serrated adenoma, S superficially serrated adenoma, N normal mucosa
