Fig. 1
From: Alternative PDGFD rearrangements in dermatofibrosarcomas protuberans without PDGFB fusions
Cryptic COL1A1-PDGFB fusion in dermatofibrosarcoma protuberans. a–b Band-like infiltration of hypodermis and subcutaneous adipose tissue (case number 15, HES staining, × 2.5 and × 6.5 magnifications, respectively) c Storiform proliferation infiltrating hypodermis with a honeycomb pattern (HES, × 200 magnification). d–e High-power field view of tumor cell nuclei displaying ovoid shape and monomorphism d and focal interspersed multinucleated cells e (HES, × 200 magnification) f Fluorescence in situ hybridization (FISH) using COL1A1 break-apart probe. FISH analysis shows an unbalanced translocation with a gain of the 5′ part of COL1A1 (probe labeled in red). g Array-comparative genomic hybridization profile harbors a genomic breakpoint at COL1A1 locus with a 5′COL1A1 gain but no breakpoint at PDGFB locus (chromosome 22 view not shown). Additional copy number alterations include gains of 4q28-q35, 6p, and whole chromosome 7
