Fig. 3
From: Alternative PDGFD rearrangements in dermatofibrosarcomas protuberans without PDGFB fusions
EMILIN2-PDGFD dermatofibrosarcoma protuberans. a Biopsy specimen highlighting densely cellular tumor infiltrating hypodermis (case number 9, HES staining, × 2 magnification). b Focal collagenized stroma arranged in elongated fascicles suspicious for fibrosarcomatous transformation (× 200 magnification). c Tumor cells display ovoid monomorphic nuclei embedded in a collagenous stroma (× 400 magnification). d CD34 staining in this case (× 250 magnification). e Array-comparative genomic hybridization profile showing a homozygous deletion of CDKN2A (P16) but no breakpoint within EMILIN2 or PDGFD loci. f–g Fluorescence in situ hybridization (FISH) using EMILIN2 break-apart probe f and PDGFD break-apart probe g showing a rearrangement of the EMILIN2 and PDGFD loci, respectively. h Structure of EMILIN2-PDGFD fusion. From top to bottom scheme representing locus and chromosomal position of EMILIN2 and PDGFD; schematic of breakpoints positions which constantly occurred in exon 4 of EMILIN2 and in exon 6 of PDGFD; nucleotide sequence of adjoined sequences
