Table 3 Immunophenotypic characteristics of hairy cell leukemia-variant
Marker | Positive /Total cases studied | Bright | Dim/Partial | Negative |
|---|---|---|---|---|
Immunohistochemistry | ||||
  CD20 | 21/21 (100%) | 21 (100%) | — | — |
  PAX-5 | 13/13 (100%) | 13 (100%) | — | — |
  DBA.44 | 14/15 (93%) | 14 (93%) | — | 1 (7%) |
  Annexin-1 | 0/18 (0%) | — | — | 18 (100%) |
  Cyclin D1 | 0/10 (0%) | — | — | 10 (100%) |
  BRAF | 0/8 (0%) | — | — | 8 (100%) |
Flow Cytometry | ||||
  CD19 | 22/22(100%) | 22 (100%) | — | — |
  CD20 | 21/21(100%) | 21 (100%) | — | — |
  CD22 | 18/18 (100%) | 18 (100%) | — | — |
  CD79b | 17/17 (100%) | 13 (77%) | 4 (23%) | — |
  CD11c | 21/21 (100%) | 18 (86%) | 3 (14%) | — |
  CD103 | 21/22(95%) | 19 (86%) | 2 (9%) | 1 (5%) |
  CD25 | 3/22(14%) | — | 3 (14%) | 19 (86%) |
  CD5 | 0/22 (0%) | — | — | 22 (100%) |
  CD10 | 0/22 (0%) | — | — | 22 (100%) |
  CD23 | 0/16 (0%) | — | — | 16 (100%) |
  CD38 | 0/10 (0%) | — | — | 10 (100%) |
  CD43 | 0/10 (0%) | — | — | 10 (100%) |
  CD52 | 8/8 (100%) | 8(100%) | — | — |
  CD200 | 7/12 (58%) | — | 7 (58%) | 5 (42%) |
  FMC7 | 10/11 (91%) | 10 (91%) | — | 1 (9%) |
  CD123 | 1/1 (100%) | 1/1 (100%) | — | — |
 Surface light chains Kappa/lambda | 20/22 (91%) 10/10 | 12/19 (63%) | 7/19 (37%) | 2/22 (9%) |
