Fig. 2

Immunohistochemical staining of amphiregulin and epidermal growth factor in human biopsies (200×). The proteins of interest are stained brown in cells against the contrast of the blue hematoxylin counterstain. Amphiregulin expression is lower in gastrointestinal graft-versus-host disease samples, gastrointestinal acute GVHD samples without apoptotic changes (non-diagnostic), and ulcerative colitis patients compared to normal colon. No significant difference in epidermal growth factor staining across patient groups is apparent