Fig. 2
Examples of prostate tissue architecture visualized using nonlinear microscopy imaging of freshly excised, unfixed specimens that were not physically sectioned versus corresponding standard paraffin embedded H&E slides after standard histological processing. a Nonlinear microscopy and b paraffin H&E of benign ducts with basal (blue arrow) and secretory (orange arrow) layers (20x objective), c nonlinear microscopy, and d paraffin H&E of a neural bundle (10x objective, 0.75 µm pixel), e nonlinear microscopy of stroma (10x objective, 0.75 µm pixel) with f corresponding paraffin H&E, and g nonlinear microscopy and h paraffin H&E of an artery (10x objective, 1 µm pixel). All scale bars are 100 µm
