Fig. 7
A nonlinear microscopy image of a freshly excised unfixed specimen and corresponding paraffin H&E slide exhibiting foamy gland variant of prostate cancer with Gleason score 6 (3 + 3). The cytoplasm of the cancer cells is less ‘foamy’ in the nonlinear microscopy images than the paraffin H&E (red, blue). Intraluminal crystalloids (yellow arrows) are present in the images. (10x objective, 0.75 µm pixel)
