Fig. 9

A white-light photograph and nonlinear microscopy image of multi-centimeter, freshly excised, unfixed prostate tissue with tumor touching the blue ink. The tumor can be seen touching the ink in the nonlinear microscopy image (red and blue box, green arrows). The paraffin H&E slide did not show tumor touching the ink because of discrepancy between the nonlinear microscopy imaging plane and paraffin embedded H&E section. (10x objective, 1 µm pixel)