Fig. 1
From: GLI1-amplifications expand the spectrum of soft tissue neoplasms defined by GLI1 gene fusions
Pathologic and FISH findings of GLI1-amplified tumors. A-B: Images from Case 1 (4/F, shoulder). A well-circumscribed lesion with distinct lobulated architecture (A) showing monomorphic ovoid cells arranged in a cords separated by delicate capillaries (B). C-I: Images from Case 2 (10/M, finger) of a superficial dermal nodule with a nodular growth pattern (C), in areas protruding into dilated vascular spaces (D) and showing tumor cells with clear cytoplasm in a nested arrangement (E). Immunohistochemical stains for CDK4 (F) and MDM2 (G) were strongly positive. FISH studies showing (H) amplification and break-apart of GLI1 gene (green- 5’-centromeric separated from red-3’-telomeric, arrow; the latter red signal being also being amplified) and (I) Co-amplifications of CDK4 and MDM2 genes (orange-MDM2, red-CDK4, arrow)
