Table 1 Immunohistochemical staining details
Marker | Supplier | Clone | Antigen retrieval | Primary dilution | Incubation | Platform |
|---|---|---|---|---|---|---|
SATB2 (1) | Abcam | SATBA4B10 | 30 min HIER using high pH Tris buffer | 1:400 | 30–10M-30 | DAKO Omnis |
SATB2 (2)* | Abcam | EPNCIR130A | 30 min HIER using high pH Tris buffer | 1:800 | 30–10R-30 | DAKO Omnis |
PAX8 | Cell Marque | MRQ-50 | 30 min HIER using high pH Tris buffer | 1:200 | 30–10R-30 | DAKO Omnis |
CK7 | Dako | OV-TL 12/30 | 30 min HIER using high pH Tris buffer | RTU | 12 × 20 | DAKO Omnis |
CK20 | Dako | KS20.8 | 30 min HIER using high pH Tris buffer | RTU | 25 × 20 | DAKO Omnis |
CDX2 | Dako | DAK-CDX2 | 30 min HIER using high pH Tris buffer | RTU | H10–10M-10 | DAKO Omnis |
