Fig. 4
From: Absence of H3F3A mutation in a subset of malignant giant cell tumor of bone
H3F3A copy number analysis using FISH (lung metastasis in case 6 that was illustrated in Fig. 3). Top (outlined in red): The H3F3A G34W-positive giant cell poor component showing intact copies of the H3F3A gene (i.e., two copies of H3F3A [red] and two copies of CEN1q [green]; a H3.3 G34W immunohistochemistry; b FISH). Bottom (outlined in blue): The H3F3A G34W-negative giant cell-rich component showed the monoallelic loss of H3F3A (i.e., one copy of H3F3A [red] and two copies of CEN1q [green]; c H3.3 G34W immunohistochemistry; d FISH)
