Table 1 Antibody characteristics and used protocol for whole tissue IHC
From: Ductal carcinoma in situ of the breast: immune cell composition according to subtype
Antibody* | Cell type | Type | Concentration | Company | Clone | Lot number | Procedure | Incubation time |
|---|---|---|---|---|---|---|---|---|
ER | n.a. | Anti-rabbit | 1 µg/ml | Ventana | SP1 | F02583 | Ultraview CC1 64′ | 32 min |
PR | n.a. | Anti-rabbit | 1 µg/ml | Ventana | 1.00E+02 | Y08684 | Ultraview CC1 36′ | 12 min |
HER2neu | n.a. | Anti-rabbit | 6 µg/ml | Ventana | 4B5 | E06192 | Ultraview CC1 36′ | 32 min |
CD4 | T-helper cell | Anti-rabbit | 2.5 µg/ml | Ventana | SP35 | G07304 | Ultraview CC1 64′ | 8 min |
CD8 | Cytotoxic T cell | Anti-rabbit | 0.35 µg/ml | Ventana | SP57 | Y04591 | Optiview CC1 16′ | 32 min |
CD20 | B cell | Anti-mouse | 0.3 µg/ml | Ventana | L2G | Y19660 | Ultraview CC1 64′ | 44 min |
CD68 | Macrophage | Anti-mouse | 0.4 µg/ml | Ventana | KP1 | G01685 | Optiview CC1 16′ | 8 min |
FOXP3 | Regulatory T-cell | Anti-mouse | 0.5 mg/ml | Thermofisher | 236A/E7 | 4339062 | Optiview CC1 32′ | 32 min |
PDL1** | n.a. | Anti-rabbit | 7 µg/ml | Ventana | SP142 | Y03641 | Optiview CC1 64′ | 16 min |
PDL1** | n.a. | Anti-rabbit | 1.61 µg/ml | Ventana | SP263 | Y2898U | Optiview CC1 64′ | 16 min |
