Fig. 2 | Modern Pathology

Fig. 2

From: Mapping the binding sites of antibodies utilized in programmed cell death ligand-1 predictive immunohistochemical assays for use with immuno-oncology therapies

Fig. 2

Epitope mapping of r22C3 and r28-8 anti-PD-L1 antibodies by hydrogen/deuterium exchange mass spectrometry. a Hydrogen/deuterium exchange mass spectrometry differential plots of antigen-binding fragments (Fab) of r22C3 and r28-8 antibodies showing mean difference in deuterium uptake between the antibody-bound state of the PD-L1 extracellular domain and the unbound (Apo) extracellular domain averaged across all time points. In the presence of antibody, residues that demonstrate differences show less deuterium uptake due to protection from exchange by the bound antibody. b Molecular modeling illustrating PD-L1 epitopes identified for r22C3 and r28-8 antibodies by hydrogen/deuterium exchange mass spectrometry. Epitope segments with strong and modest protection are in dark and light blue, respectively. This structural figure was generated using PyMOL with coordinates taken from PDB accession number 3FN3. Fab fragment antigen-binding, PDB Protein Data bank, PD-L1 programmed cell death ligand-1, r22C3 recombinant 22C3, r28-8 recombinant 28-8

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