Fig. 4

Immunohistochemical staining using PD-L1 antibodies. H-scores and representative images of Expi293F cell lines transfected with a PD-L1 WT, Int1, Int2, Int3, Int4, Int5, Int6, Int7, WT PD-L2, and PD-L2-PD-L1 fusion constructs stained with SP263 and SP142 assays and E1L3N laboratory-developed test. b H-scores and representative images of Expi293F cell lines transfected with PD-L1 WT, Ext1, Ext2, Ext3, Ext4, Ext5, Ext6, Ext7, Ext8, Ext9, Ext10, Ext11, Ext12, and Ext13 constructs stained with 22C3 and 28-8 assays. Proportion of total H-score contributed by cells staining at each intensity is represented by red (3+), orange (2+), and green (1+). c Immunohistochemical staining of PD-L2 WT and P2-P1 transfected cell lines with SP263, SP142, E1L3N, 22C3, 28-8, and PD-L2 antibodies. PD-L1 programmed cell death ligand-1, PD-L2 programmed cell death ligand-2, P2-P1 construct containing the extracellular domain of PD-L2 fused to the last 27 residues of the PD-L1 cytoplasmic domain, untrans untransfected, WT wild-type