Fig. 4 | Modern Pathology

Fig. 4

From: Frequent overexpression of klotho in fusion-negative phosphaturic mesenchymal tumors with tumorigenic implications

Fig. 4

RNA CISH (RNAscope) to detect KL and KLB expression. a PMT-58-2 and PMT-73 showed a score of 4 for KL and KLB, respectively, while PMT-2 was negative for both genes (insets: PPIB control). Also, note the normal expression of KLB in the adjacent liver tissue in PMT-58-2 (asterisk). b Group C as a whole expressed significantly higher levels of KL than Groups A and B combined. c The KL expression levels derived from RNAscope and AMP-seq (ArcherDx) highly correlated with each other. d With two of the KL-negative Group C cases (PMT-73 and PMT-74) highly expressing KLB instead, Group C also revealed significantly higher sums of KL and KLB scores compared with Groups A and B. e The overexpression (when defined as a score of 2~4) of either gene appeared mutually exclusive (cases with scores of zero in both genes were not shown)

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