Table 2 Results of AMP-based RNA sequencing (ArcherDx)
PMT# | Groupe | Sample age (year) | Cq value | RNA reads (×103) | % RNA/total reads | Fusion junctiona | Alternative junctiona | Number of unique start sites supporting the fusion junctionb | Number of unique supporting readsb |
|---|---|---|---|---|---|---|---|---|---|
PMT-1 | A | 4 | 29.61 | 921 | 67.4 | FN1 (23)—FGFR1 (3) | FN1 (23)—FGFR1 (4) | 24 (76) | 118 (427) |
PMT-2 | A | 3 | 28.60 | 929 | 66.5 | FN1 (22)—FGFR1 (3) | FN1 (22)—FGFR1 (4) | 37 (56) | 218 (173) |
PMT-18 | A | 17 | 33.03 | 145 | 12.9 | NP | NP | ─ | |
PMT-38 | A | 9 | 31.36 | 1171 | 68.4 | FN1 (23)—FGFR1 (3) | FN1 (23)—FGFR1 (4) | 29 (78) | 215 (730) |
PMT-41 | A | 7 | 31.54 | 893 | 61.0 | FN1 (16)—FGFR1 (4) | NP | 63 | 195 |
PMT-63 | A | 1 | 26.11 | 965 | 74.2 | FN1 (24)—FGFR1 (3) | FN1 (24)—FGFR1 (4) | 333 (355) | 3938 (1673) |
PMT-64 | A | 0 | 26.04 | 923 | 73.5 | FN1 (23)—FGFR1 (9) | FN1 (23)—FGFR1 (9)c | 128 (10) | 1070 (11) |
PMT-31 | B | 11 | 34.56 | 347 | 37.9 | FN1 (23)—FGF1 (2) | NP | 9 | 71 |
PMT-50 | B | 1 | 28.51 | 1036 | 71.6 | FN1 (24)—FGF1 (2) | FN1 (24)—FGF1 (2) | 120 (78) | 1001 (281) |
PMT-10 | A > C | 3 | 27.40 | 969 | 66.6 | NP | NP | ─ | ─ |
PMT-13 | C | 9 | 31.19 | 738 | 60.0 | NP | NP | ─ | ─ |
PMT-34 | C | 6 | 30.13 | 867 | 74.1 | NP | NP | ─ | ─ |
PMT-44 | C | 7 | 31.17 | 685 | 61.3 | NP | NP | ─ | ─ |
PMT-52 | C | 3 | 28.74 | 979 | 75.1 | NP | NP | ─ | ─ |
PMT-61 | C | 2 | 26.82 | 935 | 77.6 | NP | NP | ─ | ─ |
PMT-25 | D | 1 | NP | 559 | 33.5 | NP | NP | ─ | ─ |
PMT-26 | D > B | 7 | 33.09 | 623 | 56.4 | FN1 (26)—FGF1 (2) | NP | 7 | 16 |
PMT-27 | D | 6 | NP | 24 | 2.8 | THRB (11)d—FGF10 (1) | NP | 4 | 18 |
PMT-28 | D | 19 | NP | 4 | 69.2 | NP | NP | ─ | ─ |
PMT-46 | D | 8 | NP | 451 | 64.4 | NP | NP | ─ | ─ |
PMT-48 | D | 7 | NP | 353 | 37.5 | NP | NP | ─ | ─ |
PMT-51 | D | 7 | 33.73 | 595 | 56.6 | NP | NP | ─ | ─ |
PMT-58 | D > C | 4 | 31.14 | 807 | 74.9 | NP | NP | ─ | ─ |
