Fig. 1: Development of monoclonal antibodies to the chimeric protein PAX3-FOXO1. | Modern Pathology

Fig. 1: Development of monoclonal antibodies to the chimeric protein PAX3-FOXO1.

From: Immunohistochemical detection of PAX-FOXO1 fusion proteins in alveolar rhabdomyosarcoma using breakpoint specific monoclonal antibodies

Fig. 1

A The transcription factor PAX3 contains a paired boxed domain (PB), homeodomain-DNA-binding domain (HD) and a PAX3 transactivation domain. FOXO1 contains a forkhead DNA-binding domain (FD) and a transactivation domain. The sequence of peptide PF1 spanning the fusion junction site is shown with the PAX3 amino acids in gray and the FOXO1 amino acids in black. The corresponding PAX7-FOXO1 sequence is shown with identical amino acids underlined. Adapted from [24, 25]. B Recognition of PAX3-FOXO1 by anti-PAX3-FOXO1 peptide mAbs. PAX3-FOXO1 was immunoprecipitated specifically from cellular lysate from the FP-RMS cell line RH-30 by anti-PAX3-FOXO1 mAbs PFM.1 (lane 2) and PFM.2 (lane 3), but not by IgG1 control mAb MOPC21 (lane 1) or by anti-NCOA3 peptide mAb AC3 (lane4). Immunoprecipitated proteins were separated by SDS-PAGE, transferred to nylon membranes and detected using anti-FOXO1 antibodies.

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