Fig. 2: Universal cRASL-seq assay for pathogen-associated RNA analysis. | Modern Pathology

Fig. 2: Universal cRASL-seq assay for pathogen-associated RNA analysis.

From: Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping

Fig. 2

Each reference organism was serially diluted into PBS and added directly to the lysis buffer and probe pool. NLC, No Ligase Control; NTC, No Template Control. The extraction free protocol of Fig. 1C was performed with all 80 probe sets in a single pool. Molecular Equivalents are calculated by normalizing read counts to a PCR spike-in sequence of known copy number. Detection of aggregate probes' read counts at a signal >10× the NTC was used to calculate the assay’s limit of detection for each organism.

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