Table 1 Pan-pathogen detection using cRASL-seq.

From: Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping

Organism

Limit of detection

Candida albicans

150 cfu/mL

Cryptococcus neoformans

1.5 cfu/mL

Mycobacterium smegmatis

150 cfu/mL

Staphylococcus aureus

150 cfu/mL

Pseudomonas aeruginosa

1.5 cfu/mL

Haemophilus influenzae

1.5 cfu/mL

Human cytomegalovirus

1.5 pfu/mL

Zika virus

15 pfu/mL

  1. Limits of detection using the multiplex cRASL-seq assay. Bacterial, fungal, or viral stocks were serially diluted in 1x-PBS, pH 7.4 and used in a cRASL-seq assay together with a multiplex probe pool targeting all organisms (Candida albicans, Cryptococcus neoformans, Mycobacterium smegmatis, Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenzae, Cytomegalovirus, Zika virus). Limits of detection were calculated as the serial dilution with a signal that was 10× above no template controls.