Fig. 1: Telomere length analysis by FISH in pituitary adenomas.

Two representative examples of cases showing either short or normal telomere lengths in tumor cells are shown. a This case shows strikingly diminished telomere signals in tumor cells (asterisks) as compared to the entrapped non-neoplastic cells (arrows). b This case displays comparable telomere intensities in tumor cells (asterisks) with those observed in the entrapped non-neoplastic cells (arrows). In both images, the DNA is stained with DAPI (blue) and telomere DNA is stained with the Cy3-labeled telomere-specific peptide nucleic acid probe (red). It is noteworthy that the centromere DNA, stained with the FITC-labeled centromere-specific peptide nucleic acid probe, has been omitted from the image to emphasize the differences in telomere lengths. Original magnification, ×400.