Fig. 2: ALT-positive pituitary adenomas and ATRX and DAXX immunostaining.
From: Telomere length alterations and ATRX/DAXX loss in pituitary adenomas
Representative regions of the three cases (15, 95, and 111) are shown. a, d, g Representative images of ALT-positive cases displaying ultrabright telomeric FISH signals as indicative of ALT. The DNA is stained with DAPI (blue) and telomere DNA is stained with the Cy3-labeled telomere-specific peptide nucleic acid probe (red). b, e, h Case 95 shows tumor cell-specific loss of nuclear ATRX expression, whereas cases 15 and 111 display intact ATRX nuclear expression. (Original magnification ×400). c, f, i Case 15 shows tumor cell-specific loss of nuclear DAXX expression, whereas cases 95 and 111 display intact DAXX nuclear expression. For all images presented—original magnification, ×400.
