Table 3 Immunohistochemical and molecular genetic findings in seven patients with PTC-DTF and PTC-NFS.
Case | Myofibroblastic stroma IHC | PTC IHC | Molecular |
|---|---|---|---|
1 | Beta Catenin + (Nuclear) Keratin AE1/AE3− MIB1 < 5% PAX8− P53− SMA+ | Beta Catenin + (Membranous) Keratin AE1/AE3+ MIB1 < 5% PAX8+ P53− SMA− | CTNNB1 c.121A>G, (p.Thr41Ala) |
2 | Beta Catenin + (Nuclear) Keratin AE1/AE3− MIB1 < 5% PAX8− P53− SMA+ | Beta Catenin + (Membranous) Keratin AE1/AE3+ MIB1 < 5% PAX8+ P53− SMA− | CTNNB1 c.133T>C, (p.Ser45Pro) Epithelial component not available for testing, however previously tested [13] and positive for BRAF c.1799T>A (p.Val600Glu) |
3 | Beta Catenin + (Nuclear) Keratin AE1/AE3− MIB1 < 5% PAX8− P53− SMA+ | Beta Catenin + (Membranous) Keratin AE1/AE3+ MIB1 < 5% PAX8+ P53− SMA− | CTNNB1 c.124A>G, (p.Thr41Ala) |
4 | Beta Catenin + (Nuclear) Keratin AE1/AE3− MIB1 < 5% PAX8− P53− SMA+ | Beta Catenin + (Membranous) Keratin AE1/AE3+ MIB1 < 5% PAX8+ P53− SMA− | Failed repeat sequencing/QNS, However, epithelial and mesenchymal component previously tested and reported positive [13] for: CTNNB1 c.133T>C (p.Ser45Pro) BRAF c.1799T>A (p.Val600Glu) |
5 | Beta Catenin − (Nuclear) Keratin AE1/AE3− MIB1 ~5–10% PAX8− P53− SMA+ | Beta Catenin + (Membranous) Keratin AE1/AE3+ MIB1 < 5% PAX8+ P53− SMA− | BRAF c.1799T>A, (p.Val600Glu) USP6 rearrangement negative by NGS |
6 | Tissue exhausted | Tissue exhausted | CTNNB1 c.121A>G, (p.Thr41Ala) NRAS c.175G>A, (p.Ala59Thr) |
7 | Beta Catenin + (Nuclear) Keratin AE1/AE3− MIB1 < 5% PAX8− P53− SMA+ | Beta Catenin + (Membranous) Keratin AE1/AE3+ MIB1 < 5% PAX8+ P53− SMA− | CTNNB1 c.134C>T, (p.Ser45Phe) BRAF c.1799T>A, (p.Val600Glu) |
