Fig. 1: Detection of EBV genome in lymphoma cases by real-time quantitative PCR.

Serial dilution of Namalwa DNA containing 500,000 to 0.5 copies of EBV genomes were amplified using primer/probe combinations specific for EBV EBNA1 (a) and BamHI-W conserved region (b) and the related standard curve was used to quantify the two genes segments in lymphoma cases of the indicated histology. The y-intercept corresponds to Ct values while the x-intercept corresponds to the copy number of each target expressed in log 10 scale.