Fig. 5: RNAscope and clonality analyses of an EBV-positive cHL case relapsed as EBV-negative by conventional methods (a–e) and an EBV-positive cHL case evolved to a lymphoma intermediate between cHL and DLBCL EBV-negative by conventional methods (f–j). | Modern Pathology

Fig. 5: RNAscope and clonality analyses of an EBV-positive cHL case relapsed as EBV-negative by conventional methods (a–e) and an EBV-positive cHL case evolved to a lymphoma intermediate between cHL and DLBCL EBV-negative by conventional methods (f–j).

From: Frequent traces of EBV infection in Hodgkin and non-Hodgkin lymphomas classified as EBV-negative by routine methods: expanding the landscape of EBV-related lymphomas

Fig. 5

Clonal EBV infection was detected by EBER-ISH in the onset biopsy of a cHL case (blue chromogen; a). Conversely, EBER-ISH staining resulted negative in the relapse biopsy post-therapy (b). However, occasional red dots indicating EBNA1 mRNA were observed in rare tumor cells of the relapse biopsies (panel c). An identically sized clonal immunoglobulin heavy chain variable gene rearrangement (266 base pairs) was identified in HRS cells microdissected from both the initial (panel d) and the relapse (e) biopsies. EBER-ISH positive cHL (blue chromogen; f) evolved in EBER-ISH-negative lymphoma with features intermediate between cHL and DLBCL (g). Occasional red dots indicating EBNA1 mRNA were observed in rare tumor cells of the relapse biopsies (h). An identically sized clonal immunoglobulin heavy chain variable gene rearrangement (262 base pairs) was identified in whole-tissue sections from both the initial (i) and the relapse (j) biopsies.

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