Fig. 3: A typical case of gastric MANEC displays gain of CCNE1 and loss of FAT1 in both the NEC component and the adenocarcinoma component. | Modern Pathology

Fig. 3: A typical case of gastric MANEC displays gain of CCNE1 and loss of FAT1 in both the NEC component and the adenocarcinoma component.

From: Chromosomal and molecular pathway alterations in the neuroendocrine carcinoma and adenocarcinoma components of gastric mixed neuroendocrine–nonneuroendocrine neoplasm

Fig. 3

a The gain of 19q12 (CCNE1, down arrow) and loss of 4q35.2 (FAT1, up arrow) were observed in both components in the whole genome view. b FISH analysis detected multiple copies of CCNE1 (orange) in both components (top panels) and the loss of the FAT1 gene (orange) in both components (lower panels). c qPCR validation of CCNE1 and FAT1 in 18 gastric MANECs. The CN of CCNE1 was significantly higher in NEC and ADC components than in normal tissue, and the CN of FAT1 was significantly lower in NEC and ADC components than in normal tissue. There was no difference in the CN of CCNE1 and FAT1 between NEC and ADC components. d Immunohistochemistry staining (200×) showed diffusely positive expression of cyclin E (the protein product of CCNE1) in both components but negative expression in normal mucosa. FAT1 was negative in both tumor components but positive in normal mucosa.

Back to article page