Fig. 6: A typical case of gastric MANEC displayed loss of MAPK1 only in the NEC component. | Modern Pathology

Fig. 6: A typical case of gastric MANEC displayed loss of MAPK1 only in the NEC component.

From: Chromosomal and molecular pathway alterations in the neuroendocrine carcinoma and adenocarcinoma components of gastric mixed neuroendocrine–nonneuroendocrine neoplasm

Fig. 6

a The loss of 22q11.21 (including MAPK1, black arrow) was much more significant in the NEC component than in the ADC component in the whole genome view. b FISH analysis showed loss of the MAPK1 gene (one orange signal) in the NEC component and diploid signals (pairs of orange signals) in the ADC component. c qPCR validation of MAPK1 in 18 gastric MANECs. The CN of MAPK1 was significantly lower in the NEC component than in the ADC component and normal tissue. d Immunohistochemistry staining (200×) showed the negative expression of ERK2 (the protein product of MAPK1) in the NEC component and the positive expression of ERK2 in the ADC component and normal mucosa.

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