Table 2 Immunohistochemical results.
Case | Keratins | KIT/DOG1 | SMARCB1/SMARCA4 | NUT | Other positive markers | Other negative markers |
|---|---|---|---|---|---|---|
1 | Rare cells AE1/AE3-positive; OSCAR and 34βE12-negative | Negative | Both retained | Uniformly positive; speckled nuclear pattern | CD34 smooth muscle actin (<25% of cells) | Desmin, S100 protein, SDHB (normal), MUC4, STAT6 |
2 | Uniformly AE1/AE3-positive; rare cells OSCAR-positive. 34βE12-negative | Negative | Both retained | Uniformly positive; speckled nuclear pattern | Smooth muscle actin (<25% of cells, weak) ERG protein (<25% of cells, weak) | HMB45, desmin, S100 protein, CD34, synaptophysin, Chromogranin, CD56, K5, K7, K20, STAT6, smooth muscle myosin, calponin, ALK, myogenin, CD31, calretinin, MyoD1, TRK |
3 | >50% of cells AE1/AE3-positive | Negative | Both retained | Uniformly positive; speckled nuclear pattern | Synaptophysin (<25% of cells, weak) | Chromogranin, CD45, S100 protein, desmin, WT1, calretinin, K7, K20, PAX8, DOG1, CD34, ERG, OCT4, CD68, smooth muscle actin, myeloperoxidase, MDM2, HMB45, MelanA |
4 | Rare cells AE1/AE3-positive; OSCAR and 34βE12-negative | Negative | Both retained | Uniformly positive; speckled nuclear pattern | CD99 (<25% of cells, weak) | STAT6, CD34, HMB-45, BCL-2, S100 protein, SOX10, CD45, CD56, CD21, D2–40, ALK, CD10, calretinin, smooth muscle actin, desmin, caldesmon, ERG, inhibin, WT1, PAX5, PAX8, TdT, BRAFv600E, MDM2,TRK |
5 | Rare cells AE1/AE3-positive; OSCAR and 34βE12-negative | Negative | Both retained | Uniformly positive; speckled nuclear pattern | CD99 (perinuclear dot like) | CD34, desmin, smooth muscle actin, S100 protein, SOX-10, WT1, K8/18, K6/6, CD45, MelanA, HMB-45, Alk-1, Calretinin, TDT, Inhibin, PAX-8, ER, p40, CD56, synaptophysin |
