Table 3 Mutations and fusions detected by DNA and RNA sequencing.

From: Re-evaluating tumors of purported specialized prostatic stromal origin reveals molecular heterogeneity, including non-recurring gene fusions characteristic of uterine and soft tissue sarcoma subtypes

Case	Diagnosis	Morphologic pattern^a	TMB	Mutations/indels (DNA SEQ)	Rearrangements (DNA SEQ)	RNA SEQ	Fusions
1	PSS	Patternless	Nab	None found	NAB2-STAT6	Yes	NAB2-STAT6
2	PSS	Short fascicles	6.1	None found	TPM3-NTRK1	No	NA
3	PSS	Patternless/Epithelioid	6.1	POLQ p.S1694*	BCOR	Yes	BCOR-MAML3
4	PSS	Epithelioid/Storiform	6.1	ATRX p.Y1850*	TP53	Yes	Failed
5	PSS	Patternless/Epithelioid	9.9	TP53 p.E343*	None found	Yes	Failed
				ATRX p.K2158Nfs9*	None found
6	PSS	Short fascicles/Storiform	6.1	RASA1 p.Y528Cfs4*	None found	No	NA
				PTEN p.T319*	None found
7	PSS	Fibrosarcomatous/Soriform	4.6	KAT6B p.E1204Gfs14*	None found	Yes	None found
				SUFU p.E130Vfs39*	None found
8	PSS	Short fascicles	8.4		None found	Yes	None found
				BCORL1 p.L1287Afs29*	None found
9	PSS	Short fascicles/Patternless	8.4	ARID1A p.R2116Tfs33*	None found	Yes	None found
				ARID1A p.R1989*	None found
				ARID1A p.V1058Efs46*	None found
10	PSS	Short fascicles	NA	NA (failed sequencing/low DNA)	NA (failed sequencing/low DNA)	No	NA
11	PSS	Patternless/Epithelioid^c	6.1^c	TP53 p.P117R	None found	No	NA
				CHEK2 p.T367Mfs*15
	STUMP	Myxoid^c	6.1^c	TP53 p.P117R	None found	No	NA
				CHEK2 p.T367Mfs*15
12	STUMP	Patternless	3.8	BCOR p.E1151*	JAZF1-SUZ12	No	NA
13	STUMP	Phyllodes	2.3	None found	RGPD2-FOXO1	Yes	None found
14	STUMP	Degenerative atypia	2.3	TP53 p.G244D	CLOCK-TP53	Yes	CIC^d
					PALB2
15	STUMP^e	Phyllodes	NAb	TP53 p.A159P	None found	No	NA
16	STUMP	Phyllodes	3.8	TP53 p.N131del	None found	Yes	None found
				ATR p.Q31*	None found
17	STUMP	Phyllodes	5.3	KMT2D p.E4025K	None found	Yes	None found
18	STUMP	Hypercellular	9.1	RSPO p.M16_C21del	None found	Yes	None found
19	STUMP	Hypercellular	2.3	POT1 splice site mutation	None found	Yes	ch12 events^f
20	STUMP	Myxoid	3.0	None found	None found	Yes	None found
21	STUMP	Myxoid	NA^b	None found	None found	Yes	None found
22	STUMP	Hypercellular	1.5	None found	None found	Yes	CIC-SERPINF1^d
23	STUMP	Hypercellular	NA	NA (failed sequencing/low DNA)	NA (failed sequencing/low DNA)	Yes	None found
24	STUMP	Hypercellular	NA	NA (failed sequencing/low DNA)	NA (failed sequencing/low DNA)	No	NA
25	STUMP	Phyllodes	NA	NA (failed sequencing/low DNA)	NA (failed sequencing/low DNA)	No	NA

ch chromosome, NA not available/applicable, SEQ sequencing, STUMP stromal tumor of uncertain malignant potential, PSS prostatic stromal sarcoma, TMB tumor mutational burden.
^aAs descibed in Herawi et al. [1].
^bTMB could not be calculated due to low mutational load or technical reasons.
^cIn the manuscript, this case was counted as a PSS; therefore, only the histopathologic features and TMB of the PSS component were quantified.
^dThese events were found at a low frequency and might be stochastic in nature.
^eThis case was initially diagnosed as STUMP, but later developed intraperitoneal metastases.
^fThese alterations in chrmosome 12 are usually surrogate markers of MDM2 amplification, as previously described by Dickson et al. [14].

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Table 3 Mutations and fusions detected by DNA and RNA sequencing.

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