Fig. 3: Relative ALT-associated telomere focus size based on ATRX or SMARCAL1 status.
From: SMARCAL1 loss and alternative lengthening of telomeres (ALT) are enriched in giant cell glioblastoma
Representative telomere (red) and centromere (green) FISH images from A an ALT-negative giant cell GBM case, and from ALT-positive giant cell GBM cases with either B ATRX loss or C SMARCAL1 loss (original magnification ×400). Scale bar equals 50 microns and the arrows point to ultrabright telomeric FISH signals present in the ALT-positive cases. D Focus size was measured for all ALT-positive giant cell GBM cases under constant exposure, and focus sizes were compared. A cutoff of 75 pixels2 (red line) was chosen for comparative analyses.
