Fig. 2: Characterization of immune contextures.
From: Spatial immunoprofiling of the intratumoral and peritumoral tissue of renal cell carcinoma patients
A Panel design used in multiplex immunohistochemistry (mIHC). GFP, Cy3, Cy5, and Cy7 represents fluorescence channels and Chromo1 and Chromo2 chromogenic channels. B Representative fluorescence and chromogen staining between intratumoral (IT) and peritumoral (PT) tissues. C Contexture-level heatmap of immunophenotypes in IT, PT, and control tissues with median-averaged immune cell proportions. Clustering has been computed with the Euclidean distance of immunophenotype correlations (Spearman). D Spearman correlation between IT and PT immunophenotypes. Significance: ***p < 0.001, **p < 0.01, *p < 0.05. E Patient-level heatmap of immunophenotypes in IT and control tissues. Clustering has been computed with the Euclidean distance of immunophenotype correlations. F Balloonplot visualizing the log10 fold-change difference of immunophenotype proportions between immune topographies. Each topography has been compared to the pooled group of other topographies. The balloon size corresponds to the p-value (Wilcoxon test). Only immunophenotypes differing in any comparison (p < 0.05) are shown.
