Fig. 1: Study Design. | Modern Pathology

Fig. 1: Study Design.

From: Digital validation of breast biomarkers (ER, PR, AR, and HER2) in cytology specimens using three different scanners

Fig. 1

Total of 96 glass slides were scanned; including 20 cell blocks H&E; 20, ER; 20, PR; 16, AR; and 20, HER2 stained immunohistochemistry slides three times on each scanner. The review was conducted in 2 phases, first, each of the four pathologists (Pathologist A-D) randomly reviewed a total of 15 digital cases, with their corresponding markers, 5 cases from each scanner on 3 different occasions, at least 14 days apart. Second phase was conducted 6-months after, two out of four pathologists (Pathologist B and C) reviewed all 20 digital cases with their corresponding IHC markers on each scanner to test inter-instrument concordance rate between scanners and investigate inter-observer variability. ER Estrogen Receptor, PR Progesterone Receptor, AR Androgen Receptor, HER2 Human Epidermal Growth Receptor 2, H&E Hematoxylin and Eosin stain.

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