Fig. 4: IL-6 expression by RNA ISH is elevated in MCD.
From: Transcriptome and unique cytokine microenvironment of Castleman disease
A H&E and CD138(brown)/IL-6 (red) from a reactive Castleman–like lymph node case 27) (top panel), UCD case 9 (middle panel) and MCD case 25 (bottom panel). Increased IL-6 expression along interfollicular vascular structures and lack of co-expression with CD138 in MCD. IHC at 100× and 400× magnification are shown. B Spatial differences in IL-6 expression in CD and controls. Left: IL-6 expression was quantified within each lymph node region and compared across UCD, MCD, and reactive lymph nodes. IL-6 expression was significantly increased in the interfollicular space of MCD. Middle: IL-6 expression (red) in endothelial cells and lack of co-expression with histiocytic marker CD163 (MCD case 23) Right: IL-6 co expressed with endothelial marker CD31 (right, case 25) in the interfollicular hypervascular areas. IHC at 600× magnification are shown. *P < 0.05.
