Fig. 5: VEGF expression by RNA ISH is elevated in iMCD germinal centers and interfollicular areas.
From: Transcriptome and unique cytokine microenvironment of Castleman disease
A Top panel. H&E, and CD21(brown)/VEGF(red) from a reactive Castleman –like lymph node case 27 (upper panel), UCD case 9 (middle panel) and a MCD case 25 (bottom panel). IHC at 100× and 400× magnification are shown. Increased VEGF expression in the interfollicular areas and FDC-rich germinal centers of MCD. Scattered interfollicular expression in histiocytic cells with cytoplasmic projections. B VEGF expression by RNA ISH was elevated in iMCD germinal centers and interfollicular areas. Left and middle panels: VEGF expression by RNA ISH was significantly stronger in MCD in cells within the interfollicular space and germinal center. Right panel: Co-expression of histiocyte specific marker CD163 (brown IHC) with VEGF (red, RNA ISH) in MCD case 23. IHC at 600× magnification are shown.
