Fig. 6: IGH and TRB gene rearrangement analysis.
From: Transcriptome and unique cytokine microenvironment of Castleman disease
A Clone size rank plots of TRB gene rearrangement. TRB gene rearrangements were grouped into clonotypes (see methods) and ranked by copy number into bins corresponding to the top 10 ranked clones, 11–100, 101–1000 and >1000. The copy number fractions of clones falling into the binned size ranges are shown for each individual. Total clone counts for each individual are given in parenthesis. B Clone size rank plots of IGH gene rearrangement. Expanded clones are shaded in solid black. C Distribution of somatic hypermutation (SHM) levels (percentage of mutated nucleotides, NT, away from the nearest germline IGHV gene). Data are visualized on a log10 scale to reveal the different levels of SHM between the populations. Clone counts, summed for all of the individuals in each diagnostic category, are given in parentheses. Median SHM levels are statistically significant between each population (p < 0.0001, Mann Whitney two-sided test). D Fraction of clones without SHM (< 0.5% mutation in the IGVH gene compared to the nearest germline gene).
