Fig. 1: Comparison of tumor immune and stromal content evaluation by Cell DIVE and Pathologist. | Modern Pathology

Fig. 1: Comparison of tumor immune and stromal content evaluation by Cell DIVE and Pathologist.

From: Stratification of chemotherapy-treated stage III colorectal cancer patients using multiplexed imaging and single-cell analysis of T-cell populations

Fig. 1

A Cell DIVE workflow: immunofluorescence staining with overlaying segmentation masks and resulting classification. Based on the classification data, an immune and stroma score was calculated per TMA core. B The immune score was calculated from the immune cell density as counts of segmented cells that were positive for any of the immune markers (CD45, CD3, CD4, CD8) in each core. The cores were grouped based on the Pathologist’s high-medium-low immune scores (x-axis) and each dot was the value of the Cell DIVE immune score (y-axis) per core. C The stroma score for each TMA core was calculated by counting segmented cells outside the epithelial mask that were negative for AE1 and immune markers and converting to ‘percent of total’ cells. The cores were grouped based on the Pathologist’s high-medium-low stroma scores (x-axis) and with each dot indicating stroma score (y-axis) per core. Statistical analysis was performed using Welch’s ANOVA and pairwise t-test (***P and ****P < 0.001 for all comparisons).

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