Fig. 1: Nonlinear microscopy (NLM) images of benign prostate needle core biopsies.
From: Real-time diagnosis and Gleason grading of prostate core needle biopsies using nonlinear microscopy
A An NLM image of glandular hyperplasia acquired at the biopsy surface (left), at 10 µm below the biopsy surface (center), and a corresponding formalin-fixed paraffin-embedded (FFPE) H&E section (right). The surface of biopsies can often appear hypercellular and can be difficult to interpret. B Benign glands with a basal and secretory cell layers visualized in an NLM image (left) and a corresponding FFPE H&E section (right). C Benign glands with a large corpora amylacea (NLM: left, FFPE H&E: right). D Atrophy/post atrophic basal cell hyperplasia (NLM: left, FFPE H&E: right). E Atrophy/post atrophic basal cell hyperplasia (bottom) with a glandular secretory cell component (top) (NLM: left, FFPE H&E: right). Scale bars = 50 µm.
