Fig. 6: Nonlinear microscopy (NLM) images of mucin-producing prostatic adenocarcinoma from prostate needle core biopsies.
From: Real-time diagnosis and Gleason grading of prostate core needle biopsies using nonlinear microscopy
A An NLM image (left) and a corresponding formalin-fixed paraffin-embedded (FFPE) H&E section (right). Mucin does not stain in NLM images and therefore the blue tinge seen in the FFPE H&E is not present in NLM. In NLM evaluation of fresh tissue, the mucin covers the surface of the biopsy (dashed oval) and thus occludes NLM imaging. This occlusion appears as lighter regions (dashed circle) throughout the biopsy. B NLM images acquired at the surface and 5 µm and 10 µm below the surface of the biopsy shown in A. The cribriform pattern varies in depth. Scale bars = 50 µm.
