Fig. 8: Pathology variations when changing the depth (z axis) of nonlinear microscopy (NLM) imaging.
From: Real-time diagnosis and Gleason grading of prostate core needle biopsies using nonlinear microscopy
A A nerve (dashed circle) uninvolved by carcinoma at 12 µm below the biopsy surface becomes (B) involved by carcinoma 22 µm below the tissue surface. C Large, malignant glands with intraluminal secretions adjacent to chronic inflammation and benign glands. D and E are NLM images acquired 4 µm and 8 µm below C, respectively. In C, there appears to be a potential focus of cribriform pattern (black box), which evolves into a single discrete gland in E. Scale bars = 50 µm.
