Fig. 2: Cytomorphology of testicular adult granulosa cell tumors (AGCT).

A Nuclear grooves were present in all cases (case 8 is shown in this picture). B. Atypical mitoses were identified in only one case with prominent mitotic activity (case 13). The tumor featured scattered neoplastic cells with nuclear grooves and a predominant nested architecture. This tumor had biallelic inactivation of TP53 (see sequencing results) and overexpression of p53 by immunohistochemistry (Fig. 2B, inset; Clone D-O7, mouse monoclonal, dilution 1:500; DAKO, Carpinteria, CA).