Fig. 5: Representative electropherograms of the Promega MSI Analysis System showing MSI-high by PCR and fragment analysis. | Modern Pathology

Fig. 5: Representative electropherograms of the Promega MSI Analysis System showing MSI-high by PCR and fragment analysis.

From: Detecting mismatch repair deficiency in solid neoplasms: immunohistochemistry, microsatellite instability, or both?

Fig. 5

Top panel: tumor tissue; bottom panel: matched normal tissue. The shifted alleles are indicated by arrows. Green: electropherogram showing the peaks of 2’,7’-dimethoxy-4,5-dichloro-6-carboxyfluorescein (JOE)-labeled loci, NR-21, BAT-25, and MONO-27. Blue: electropherogram showing peaks of the fluorescein-labeled loci, BAT-26 and Penta D. Black: electropherogram showing the peaks of tetramethyl rhodamine (TMR)-labeled loci, NR-24 and Penta C.

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