Fig. 1 | Molecular Psychiatry

Fig. 1

From: Adolescent exposure to Δ9-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons

Fig. 1

Model of adolescent THC exposure and approaches for layer- and cell-specific morphological and transcriptional profiling of prelimbic (PrL) pyramidal neurons. a Schematic timeline of THC exposure during adolescence and developmental time points studied. b Acute administration of 1.5 mg/kg THC increased circulating serum THC concentration 1 h after injection. c Adolescent exposure to THC elevated corticosterone levels, most notably 24 h after the last administration that may reflect a component of acute withdrawal. d–f Multidisciplinary approach to study the developmental effects of THC on pyramidal neuron morphology and gene expression in layer III of the rodent PrL. Dendritic branching d and spine density e were studied by filling pyramidal neurons with Lucifer yellow. (f) Pyramidal neurons from layer III were microdissected from Nissl-stained sections and sequenced for downstream transcriptome-wide profiling

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