Fig. 2
Overexpression of circDYM ameliorated depressive-like behavior. a Timeline of experimental procedure in the CUS-induced mouse depressive model. b–d Effects of circDYM-GFP lentivirus microinjection on the depressive-like behavior in CUS mice. One week after circControl/circDYM-GFP lentivirus microinjection, mice were exposed to a CUS or control protocol (n = 11 mice/group). SPT (b), TST (c), and FST (d) were measured after 5 weeks of CUS exposure. All data were presented as mean ± SEM. (SPT, circDYM: F(1,40) = 33.454, P < 0.001; CUS: F(1,40) = 119.263, P < 0.001; interaction: F(1,40) = 49.660, P < 0.001. TST, circDYM: F(1,40) = 4.217, P < 0.05; CUS: F(1,40) = 7.676, P < 0.01; interaction: F(1,40) = 4.795, P < 0.05. FST, circDYM: F(1,40) = 22.168, P < 0.001; CUS: F(1,40) = 91.678, P < 0.001; interaction: F(1,40) = 31.976, P < 0.001). e Timeline of experimental procedure in the LPS-induced mouse depressive model. f–h Effects of circDYM-GFP lentivirus microinjection on the depressive-like behavior in LPS mice. One week after circControl/circDYM-GFP lentivirus microinjection, mice were intraperitoneally injected with LPS (1 mg/kg, n = 11 mice/group) or saline (n = 11 mice/group) for 5 successive days. SPT (f), TST (g), and FST (h) were then measured. All data were presented as mean ± SEM. (SPT, circDYM: F(1,40) = 14.492, P < 0.001; LPS: F(1,40) = 69.880, P < 0.001; interaction: F(1,40) = 45.436, P < 0.001. TST, circDYM: F(1,40) = 6.148, P < 0.05; LPS: F(1,40) = 16.830, P < 0.001; interaction: F(1,40) = 4.140, P < 0.05. FST, circDYM: F(1,40) = 32.290, P < 0.001; LPS: F(1,40) = 32.757, P < 0.001; interaction: F(1,40) = 15.301, P < 0.001). **P < 0.01 and ***p < 0.001 vs. circControl Control group; ##p < 0.01 and ###p < 0.001 vs. circControl treated with CUS/LPS group. SPT sucrose preference test, TST tail suspension test, FST forced swim test
