Fig. 3

Prenatal TLR7 activation results in a reactive hyperactivity. a There were no changes in locomotor activity in the open field, (b) however, during the social approach test in the presence of a social partner, male IMQ-treated mice display locomotor hyperactivity. c–g To examine locomotor activity in detail, wireless telemetry devices were implanted to continuously record activity and temperature. c Double-actogram from a vehicle-treated female mouse with a 10-day baseline (12:12 h light:dark (LD), lights on zeitgeber time (ZT): 0:00, lights off: ZT 12:00, followed by 18 days of constant darkness). Note LD day 10 was excluded from analysis due to cage-change-induced activity. d Slight baseline elevation of locomotor activity in males and females following prenatal TLR7 activation. e Prenatal TLR7 activation enhanced circadian amplitude of locomotor activity. f Activity from VEH- and IMQ-treated females separated into anestrus and estrus phases demonstrating that estrus is characterized by increased activity during ZT 18–21. g Prenatal TLR7 activation led to a substantial increase in change of locomotor activity during estrus examining percent change in the activity of each mouse. *P < 0.05, **P < 0.01, ^∧P < 0.05 (main effect of VEH vs. IMQ), mean ± SEM