Fig. 3
7-Dehydrocholesterol (7-DHC) levels in the P0 brain of pups maternally exposed to vehicle or aripiprazole (ARI) (5 mg/kg). a shows changes in 7-DHC in the context of the maternal genotype. Both wild-type (WT) and Dhcr7+/− pups were grouped taking into account only their mothers’ genotype. b shows changes in 7-DHC in the context of the embryonic genotype. Pups were grouped taking into account only their own genotypes, regardless if they were born from WT or Dhcr7+/− mothers. Note that 7-DHC is increased as a result of ARI injection and it is dependent both on the maternal (a) and embryonic genotypes (b). Values were normalized against the control condition (i.e., WT-Vehicle = 1). The genotypes are depicted below the groups; Statistical significance: *p < 0.05; **p < 0.01; ****p < 0.0001. Bars correspond to the mean ± SEM. Each symbol corresponds to a single pup brain. Note that Dhcr7+/− pups have higher basal 7-DHC levels compared to their WT littermates (comparison between vehicle conditions in b), which becomes more pronounced in ARI-injected group. The raw sterol values are reported in Supplemental Material 1
