Fig. 2 | Molecular Psychiatry

Fig. 2

From: Familial t(1;11) translocation is associated with disruption of white matter structural integrity and oligodendrocyte–myelin dysfunction

Fig. 2

Efficient conversion of control and case iPS cells to oligodendroglial lineage. a1–a7 Both control and case iPS lines could be patterned to OLIG2+ precursor cells under conditions described in Methods. b1–b7 Further differentiation of cells from a gave PDFGRα+ OPCs under proliferative conditions containing FGF2 and PDGF-AA. c1–c7 Removal of mitogens produced mature oligodendrocytes that co-stained for O4 and MBP. d–f Quantification of OLIG2+ cells on day1 (d) PDFGRα+ OPCs on day7 (e) and O4+ oligodendrocytes at day21 (f) shows a significant increase in oligodendrocytes in case lines (n = 3 independent conversions for each line, unpaired t-test used to analyze p-values). Scale: a–c 50 μm

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