Fig. 6 | Molecular Psychiatry

Fig. 6

From: MicroRNA-298 reduces levels of human amyloid-β precursor protein (APP), β-site APP-converting enzyme 1 (BACE1) and specific tau protein moieties

Fig. 6

miR-298 alters APP levels in human mixed-brain culture in dose- and time-dependent fashions. a Human mixed-brain culture was treated by doses of miR-298 and NCM at doses of 0, 50, 100, 150, and 200 µM for miR-298 and at 0, 50, 100, and 200 µM for NCM. Cells were lysed and subject to western blot as described herein and visualized blots scanned for densitometry. For all doses, n = 3. b Human mixed-brain cultures were treated with a single dose (150 µM) of miR-298 or with transfection reagent containing no miRNA mimic (Mock). Culture conditioned media was harvested at 1, 2, 3, and 4 days. sAPP was measured by western blot followed by densitometry. For all time points, n = 4.

Back to article page