Fig. 3: Transcriptional profiles of resilient and susceptible MIA-exposed offspring.

Next-generation RNA sequencing was conducted in the medial prefrontal cortex (mPFC) and amygdala (Amy) of resilient (Res-POL) and susceptible (Sus-POL) MIA-exposed offspring, which were stratified based on their behavioral performance (see Fig. 3). Behaviorally characterized reference control (Ref-CON) offspring were used as a comparison. a Heat maps illustrating the clustering of differentially expressed genes (FDR: q < 0.05) in the mPFC or Amy of Sus-POL or Res-POL offspring relative to Ref-CON offspring. The color-coded key denotes upregulation (orange) and downregulation (blue) in terms of log2 fold changes. b Venn diagram denoting the number of genes that are uniquely and commonly affected in the mPFC or Amy of Sus-POL and Res-POL offspring. c Heat map of the top ten signaling pathways affected in the mPFC or Amy of Sus-POL and Res-POL offspring, as identified by Ingenuity Pathway Analysis (IPA). The color-coded key denotes the significance levels in terms of the −log(p value). d Heat map of individual genes annotated with the term “oxidative phosphorylation”. The color-coded key denotes the level of downregulation (magenta tones) in the mPFC or Amy of Sus-POL and Res-POL offspring. e Heat map of individual genes annotated with the term “DARPP-32 signaling”. The color-coded key denotes the level of upregulation (blue tones) or downregulation (magenta tones) in the mPFC or Amy of Sus-POL and Res-POL offspring. f Heat map of individual genes annotated with the term “G-protein-coupled receptor signaling”. The color-coded key denotes the level of upregulation (blue tones) or downregulation (magenta tones) in the mPFC or Amy of Sus-POL and Res-POL offspring.