Fig. 2: Generation of mice lacking AdipoR1 specifically in 5-HT neurons.
From: Modulation of depression-related behaviors by adiponectin AdipoR1 receptors in 5-HT neurons
a Schematic diagram of the strategy used for generation of ePet-CretdTomato reporter mice. b (Left panel) Representative coronal sections showing colocalization of tdTomato (red) and tryptophan hydroxylase 2 (TPH2, green) in the dorsal raphe nucleus. (Right panel) Representative coronal sections showing colocalization of tdTomato (red) and serotonin transporter (SERT, green). Scale bar = 100 μm. c Schematic diagram of generation of mice with selective deletion of AdipoR1 in 5-HT neurons. d PCR results of genotyping. e Real-time PCR analysis showing Cre-mediated deletion of exon 2 of AdipoR1 in the dorsal raphe nucleus of male and female AdipoR1f/f;ePet-Cre mice. Male: AdipoR1f/f: n = 7; AdipoR1f/f;ePet-Cre: n = 6, Female: AdipoR1f/f: n = 6; AdipoR1f/f;ePet-Cre: n = 6. ***P < 0.001 compared with AdipoR1f/f littermate controls.
