Fig. 2: Pdcd4 mediates CRS-induced synaptic plasticity impairment in hippocampus through blocking mTORC1-regulated BDNF signaling.
a Representative photomicrographs of dendritic spines from DG granular cells, scale bar, 10 μm. b Spine density in dendrites of DG granular cells in Pdcd4 KO and WT mice. n = 4 per group; mean ± SEM, two-way ANOVA and Sidak’s multiple comparison test, *P < 0.05, **P < 0.01 vs. the No-CRS group; #P < 0.05, ##P < 0.01 vs. the CRS-WT group. c Representative images of high-magnification z-stack projections of segments of the DG dendrites (scale bar, 5 µm). Mean ± SEM of spine density from control (n = 36 from four mice), OE-Pdcd4 (n = 46 from four mice). *P < 0.05 relative to control mice (unpaired two-tailed Student’s t test). d Mice hippocampus BDNF was detected by ELISA. n = 6–7 per group, Rapamycin (Rapa), mean ± SEM, unpaired two-tailed Student’s t test, *P < 0.05. e Representative photomicrographs of dendritic spines from DG granular cells, scale bar, 10 μm. f Spine density in dendrites of DG granular cells. n = 4 per group; mean ± SEM, unpaired two-tailed Student’s t test, *P < 0.05. g Quantitative RT-PCR analysis of BDNF mRNA expression in the hippocampus in Pdcd4 KO and WT mice. n = 6–7 per group; mean ± SEM, two-way ANOVA (WT vs. KO, F1,22 = 10.97, P < 0.01; No-CRS vs. CRS, F1,22 = 11.67, P < 0.01; interaction, F1,22 = 0.846, P = 0.3676) and Sidak’s multiple comparison test, *P < 0.05. h Quantitative ELISA analysis of pan-BDNF protein expression in the hippocampus in Pdcd4 KO and WT mice. n = 6 per group; mean ± SEM, two-way ANOVA (WT vs. KO F1,20 = 6.141, P < 0.05; No-CRS vs. CRS, F1,20 = 6.906, P < 0.05; interaction, F1,20 = 2.816, P = 0.1089) and Sidak’s multiple comparison test, *P < 0.05. i Quantitative ELISA analysis of pan-BDNF protein expression in the hippocampus in OE-Pdcd4 and Control mice. n = 6 per group; mean ± SEM, unpaired two-tailed Student’s t test, *P < 0.05 vs. the control group.
