Fig. 5: Pdcd4-targeting peptide treatment promotes BDNF expression and has antidepressant response.
a HEK293 cells were co-transfected with Flag-Pdcd4, HA-eIF4A and peptide. Immunoprecipitation was performed with the anti-Flag antibody. Immunoblotting was performed with anti-Flag or anti-HA antibodies. The figure represents three independent experiments that yield similar result. b HEK293 cells were transfected with BiFC plasmids. Pictures showed DAPI staining (Blue) and Venus (Yellow). Scale bar, 10 μm. c GFP antibody detected BDNF expression after transfecting each IIc-5’UTR-BDNF-GFP construct into HEK 293 cells with either TAT-NC or TAT-eIF4AVI. d The level of BDNF expression in neurons after TAT-NC or TAT-eIF4AVI administration 24 h. e ELISA detected the expression of BDNF, IL-10 and IL-6 in neurons’ medium (n = 3; **P < 0.01 compared with the TAT-eIF4AVI group). f Time course of peptide injection/CRS administration and behavior tests. g Representative photomicrographs of injection sites in the hippocampus. h Time spent in center in the open field test. n = 8–10 per group; mean ± SEM, unpaired two-tailed Student’s t test. i Time spent in open arm in the elevated plus maze. n = 8–10 per group; mean ± SEM, unpaired two-tailed Student’s t test. j Immobility time in TST, k Immobility time in FST. n = 9–11 per group; mean ± SEM, unpaired two-tailed Student’s t test, *P < 0.05 and **P < 0.01. l Sucrose consumption in SPT. n = 18 per group; mean ± SEM, unpaired two-tailed Student’s t test, *P < 0.05 and **P < 0.01.
